Abstract
Orol dengizi qurigan tubi ekstremal sho‘rlik va qurg‘oqchilik sharoitlari bilan ajralib turadi va bu hududda o‘suvchi o‘simliklarning rizosferasi ekstrimal muhitga adabtatsiya qilgan mikroorganizmlar bilan boy bo‘lishi mumkin. Ushbu tadqiqotda Orolbo‘yi hududida keng tarqalgan galofit o‘simlik —Salsola richteri ildizi va ildiz atrofidagi tuprog‘ida uchrovchi kultivirlanuvchi mikroorganizmlarni MALDI-TOF MS usuli asosida identifikatsiya qilish maqsad qilingan. Tuproq va ildiz namunalari klassik mikrobiologik usullar (bosqichli suyultirish) yordamida ajratilib, bir nechta mikroorganizmni sof kulturalari olindi. Ajratilgan izolyatlar morfologik, fiziologik hamda biokimyoviy tahlillaridan so‘ng MALDI-TOF MS tizimi orqali identifikatsiya qilindi. Natijalar rizosfera va ildiz bilan bog‘liq mikroorganizmlar tarkibida asosan Bacillus, Enterobacter oilalariga mansub bakteriyalar hamda Rhodococcus oilasiga mansub aktinomitsetlar ustunligini ko‘rsatdi. Ushbu mikroorganizmlar sho‘rlangan va qurg‘oqchil muhitga moslashgan bo‘lib, o‘simliklarning ekstremal sharoitda barqarorligini ta’minlashda muhim ahamiyat kasb etadi. Olingan natijalar Oralbo‘yi hududida fitomelioratsiya va biotexnologik maqsadlar uchun istiqbolli mikroorganizmlarni tanlashda ilmiy asos bo‘lib xizmat qiladi
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