DISTRIBUTION, TAXONOMY AND MOLECULAR-GENETIC CHARACTERIZATION OF LEPUS TIBETANUS WATERHOUSE, 1841 AND LEPUS EUROPAEUS PALLAS, 1778
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Keywords

Lepus tibetanus
Lepus europaeus
taxonomy
molecular phylogenetics
16S rRNA
GenBank
genetic distance
Uzbekistan.

How to Cite

DISTRIBUTION, TAXONOMY AND MOLECULAR-GENETIC CHARACTERIZATION OF LEPUS TIBETANUS WATERHOUSE, 1841 AND LEPUS EUROPAEUS PALLAS, 1778. (2026). Qo‘qon DPI. Ilmiy Xabarlar Jurnali, 8(9), 368-374. https://doi.org/10.70728/a.series.tab.v08.i09.047

Abstract

This paper reviews the taxonomic history and geographic distribution of Lepus tibetanus Waterhouse, 1841 and Lepus europaeus Pallas, 1778, and reports a molecular identification and phylogenetic analysis of tissue samples collected in the Navoi and Bukhara regions of Uzbekistan, based on the mitochondrial 16S rRNA gene. Genetic- distance analysis and a maximum-likelihood tree revealed low intraspecific divergence (0.00–0.49%) but markedly higher interspecific divergence (3.20–3.94%) between the two species; Uzbek specimens formed well-supported monophyletic clades with corresponding GenBank sequences (89–99% bootstrap support), confirming species-level identity. These results provide the first molecular confirmation of both taxa from Uzbekistan and contribute new data to the phylogeography of the genus Lepus in Central Asia.
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References

1. Ellerman, J.R., & Morrison-Scott, T.C.S. (1951). Checklist of Palaearctic and Indian Mammals, 1758–1946. London: British Museum (Natural History).

2. Halanych, K.M., Demboski, J.R., van Vuuren, B.J., Klein, D.R., & Cook, J.A. (1999). Cytochrome b phylogeny of the genus Lepus. Molecular Phylogenetics and Evolution, 11(2), 213–221.

3. Hall, T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

4. Hoffmann, R.S., & Smith, A.T. (2005). Order Lagomorpha. In D.E. Wilson & D.M. Reeder (Eds.), Mammal Species of the World: A Taxonomic and Geographic Reference (3rd ed.). Baltimore: Johns Hopkins University Press.

5. Kasapidis, P., Suchentrunk, F., Magoulas, A., & Kotoulas, G. (2005). The shaping of mitochondrial DNA phylogeographic patterns of the brown hare (Lepus europaeus) under the combined influence of Late Pleistocene climatic fluctuations and anthropogenic translocations. Molecular Phylogenetics and Evolution, 34(1), 55–66.

6. Katoh, K., Misawa, K., Kuma, K., & Miyata, T. (2002). MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30(14), 3059–3066.

7. Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Pääbo, S., Villablanca, F.X., & Wilson, A.C. (1989). Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences USA, 86(16), 6196–6200.

8. Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., & Higgins, D.G. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947–2948.

9. Mamat, M., Shan, W., Dong, P., Zhou, S., Liu, P., Meng, Y., Nie, W., Teng, P., & Zhang, Y. (2023). Population genetics analysis of Tolai hares (Lepus tolai) in Xinjiang, China using genome-wide SNPs from SLAF-seq and mitochondrial markers. Frontiers in Genetics, 14, Art. 1179564.

10. Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., von Haeseler, A., & Lanfear, R. (2020). IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution, 37(5), 1530–1534.

11. Ognyov, S.I. (1966). Zveri SSSR i prilezhashchikh stran. Zaitseobraznye [Mammals of the USSR and adjacent countries: Lagomorpha]. Moscow–Leningrad: Izdatel’stvo AN SSSR. (In Russian)

12. Shan, W.J., Li, Z.R., Dai, H.Y., Dong, P.C., & Zhang, Y.C. (2024). Taxonomic status of hares (Lepus spp.) in Xinjiang, China (Lagomorpha: Leporidae): An integrative approach. Zoologica Scripta, 53(3), 282–298. https://doi.org/10.1111/zsc.12645

13. Swofford, D.L. (1993). PAUP: Phylogenetic Analysis Using Parsimony, version 3.1.1. Champaign: Illinois Natural History Survey.

14. Wu, C., Wu, J., Bunch, T.D., Wang, X., & Zhang, Y. (2005). Molecular phylogenetics and biogeography of Lepus in Eastern Asia based on mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 37(1), 45–61.