Keywords
za’faron
in vitro kultura
eksplant
sterilizatsiya
ozuqa muhiti
zamburug‘li kontaminatsiya
mikroko‘paytirish
introduksiya
biotexnologiya.
How to Cite
Abstract
Mazkur maqolada Crocus sativus L. o‘simligini in vitro kulturaga kiritishning dastlabki bosqichida kuzatilgan sterilizatsiya samaradorligi va zamburug‘li kontaminatsiya holatlari tahlil qilindi. Tadqiqotda za’faronning uch xil vegetativ qismi, apikal kurtak, parenxima, ildiz disk(murtak) qismlar eksplant sifatida olinib, ular uch xil sterilizatsiya usuli bilan ishlov berildi, hamda uch xil ozuqa muhitiga ekildi. Kuzatuv natijalariga ko‘ra, barcha eksplant variantlarida turli muddatlarda zamburug‘li kontaminatsiya kuzatildi. Natijalar shuni ko‘rsatdiki, za’faronni in vitro kulturaga kiritishda eksplant manbai, yuzaki va endogen mikroflora, sterilizatsiya reagentlarining ta’sir chuqurligi hamda ozuqa muhitining tarkibi muhim omillar hisoblanadi. Tadqiqot natijalari salbiy holat sifatida emas, balki za’faron eksplantlarini sterilizatsiya qilish va kulturaga introduksiya etish protokollarini optimallashtirish zarurligini ko‘rsatadigan ilmiy ahamiyatga ega dastlabki ma’lumot sifatida baholandi. Shuningdek, kontaminatsiya manbalarini aniqlash, eksplant turini tanlash, sterilizatsiya bosqichlarini kuchaytirish va antifungal yondashuvlarni qo‘llash bo‘yicha keyingi izlanishlar uchun ilmiy tavsiyalar ishlab chiqildi.References
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