Keywords
antioksidant faollik
DPPH
ABTS
FRAP
IC₅₀
o‘simlik ekstrakti
fenol birikmalari
flavonoidlar
skrining
O‘zbekiston florasi
biotexnologiya.
How to Cite
Abstract
Mazkur maqolada O‘zbekiston sharoitida uchraydigan va amaliy jihatdan istiqbolli bo‘lgan o‘simlik xomashyolari tarkibidagi biofaol moddalarning antioksidantlik salohiyatini qiyosiy baholash, dastlabki fitokimyoviy skrining o‘tkazish hamda natijalarni statistik talqin qilish yondashuvi yoritiladi. Tadqiqot g‘oyasi o‘simliklarda to‘planadigan fenol birikmalari, flavonoidlar, taninlar, organik kislotalar, efir moylari va boshqa ikkilamchi metabolitlarning erkin radikallarni neytrallashdagi o‘rnini baholashga qaratilgan. O‘zbekiston Respublikasi Prezidentining dorivor o‘simliklarni madaniy holda yetishtirish, qayta ishlash va ulardan davolash amaliyotida keng foydalanishga doir qarorlari tabiiy xomashyoni ilmiy asosda o‘rganish, standartlashtirish va biotexnologik mahsulotga aylantirish zaruratini kuchaytiradi. Maqolada DPPH, ABTS va FRAP testlari asosida radikal inhibatsiyasi, qaytaruvchan antioksidant kuch va IC₅₀ ko‘rsatkichlarini baholash modeli taklif qilinadi. Shuningdek, maqolaga IC₅₀, DPPH, ABTS va FRAP ko‘rsatkichlari o‘rtasidagi korrelyatsion bog‘lanishni ko‘rsatuvchi statistik jadval, radikal inhibatsiya egri chiziqlari, skrining ish oqimi sxemasi va O‘zbekiston olimlari ishlari asosida shakllantirilgan GOST uslubidagi adabiyotlar ro‘yxati kiritilgan. Natijalar qismi laboratoriya protokoli asosida real tahlilga tayyorlangan hisoblash matritsasi sifatida berilgan bo‘lib, yakuniy ilmiy maqolada spektrofotometrik o‘lchovlardan olingan xom ma’lumotlar bilan almashtirilishi lozim.References
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