Vol. 8 No. 2 (2026), Series A
Vol. 8 No. 2 (2026)

PARKENT TUMANI UZUM PLANTATSIYALARIDA GRAPEVINE FANLEAF VIRUSI (GFLV)NING MOLEKULYAR DIAGNOSTIKASI

Series A
Published 2026-03-18
Sultonmurotova D.N
O‘zbekiston Milliy universiteti Toshkent, O‘zbekiston
Abdullaev A.N
O‘zR FA Genomika va bioinformatika markazi, Qibray, O‘zbekiston
Khusanov T.S
O‘zR FA Mikrobiologiya instituti, Toshkent, O‘zbekiston
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Keywords

Uzum (Vitis vinifera L.), Grapevine fanleaf virus (GFLV), fanleaf kasalligi, coat protein (CP) geni, molekulyar diagnostika, RT-PZR, elektroforez

How to Cite

PARKENT TUMANI UZUM PLANTATSIYALARIDA GRAPEVINE FANLEAF VIRUSI (GFLV)NING MOLEKULYAR DIAGNOSTIKASI. (2026). Qo‘qon DPI. Ilmiy Xabarlar Jurnali, 8(2), 139-146. https://doi.org/10.70728/a.series.v08.i02.018
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Abstract

Uzum o‘simliklarida keng tarqalgan va iqtisodiy zarar keltiruvchi Grapevine fanleaf virus (GFLV) ni molekulyar usulda aniqlash bo‘yicha tadqiqot o‘tkazildi. Namunalardan umumiy RNK ajratilib, undan cDNA sintezi amalga oshirildi. Virus mavjudligini aniqlash uchun teskari transkripsiya asosidagi polimeraza zanjir reaksiyasi qo‘llanildi. Reaksiya natijalarining ishonchliligini ta’minlash maqsadida ichki nazorat sifatida o‘simlik geniga mos primerlar ishlatildi. Olingan natijalar tekshirilgan namunalar orasida virus infeksiyasining mavjud yoki mavjud emasligini aniqlash imkonini berdi. Tadqiqot uzumchilikda virusli kasalliklarni erta diagnostika qilish va monitoring tizimini takomillashtirishda muhim ahamiyatga ega

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