Keywords
in vitro
mikroklonal ko‘paytirish
Murashige va Skoog muhiti
fitogormonlar
regeneratsiya
ildizlanish
biotexnologiya
dorivor o‘simliklar.
How to Cite
Abstract
Mazkur tadqiqotda Senna tora L. o‘simligini in vitro sharoitida mikroklonal ko‘paytirishning ilmiy asoslangan va samarali texnologiyasi ishlab chiqilgan hamda fitogormonlar kombinatsiyasining regeneratsiya va ildizlanish jarayonlariga ta’siri kompleks baholangan. Tadqiqot natijalari urug‘larning garmonsiz Murashige va Skoog (MS) ozuqa muhitida maksimal unuvchanlikka ega ekanligini ko‘rsatib, unish darajasi 100% ni, umumiy o‘rtacha unuvchanlik esa 91% ni tashkil etdi. Mikroklonal ko‘paytirish bosqichida MS + BAP (1.0 mg/L) + kinetin (0.5 mg/L) kombinatsiyasi eng yuqori regeneratsiya samaradorligini ta’minlab, ko‘payish koeffitsienti 3.5 ± 0.4 ga yetdi, bu sitokininlarning hujayra bo‘linishi va novda hosil bo‘lish jarayonidagi yetakchi rolini tasdiqlaydi. Ildizlanish bosqichida esa ½ MS + IBA (0.5 mg/L) muhitida 100% ildizlanish va rivojlangan ildiz tizimi olingan, bu auksinlarning differensiasiya jarayonlarini rag‘batlantiruvchi yuqori samaradorligini ko‘rsatadi. Olingan natijalar Senna tora o‘simligini tez, genetik jihatdan barqaror va yuqori sifatli ko‘chatlar olish imkonini beruvchi samarali biotexnologik platforma sifatida in vitro mikroklonal ko‘paytirish texnologiyasining amaliy va ilmiy ahamiyatini asoslaydi.References
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