Keywords
COVID-19
RNK viruslari
virus RNKsini ajratib olish
real-time RT-PCR
molekulyar diagnostika
TRIzol
silika spin-kolonka
magnit boncuklar
RNase kontaminatsiyasi
PCR inhibitorlari
yangi avlod sekvensiyalash (NGS)
genomik monitoring
point-of-care diagnostika..
How to Cite
Abstract
SARS-CoV-2 infection kabi RNK genomiga ega viruslar global epidemiyalar va pandemiyalarning asosiy etiologik omillaridan biri hisoblanadi. Ushbu viruslarni tezkor va aniq aniqlashda real vaqt rejimidagi revers transkripsion polimeraza zanjir reaksiyasi (real-time RT-PCR) eng ishonchli molekulyar diagnostika usullaridan biri bo‘lib, uning sezgirligi va spetsifikligi ko‘p jihatdan virus RNKsini yuqori sifatda ajratib olishga bog‘liq. Mazkur sharh maqolada SARS-CoV-2 misolida virus RNKsini ajratib olishning asosiy yondashuvlari, jumladan TRIzol asosidagi organik ekstraksiya, silika spin-kolonka va magnit boncuklarga asoslangan usullar tizimli ravishda tahlil qilindi. Shuningdek, klinik namunalarni to‘g‘ri yig‘ish, transport qilish va saqlash sharoitlari, RNase kontaminatsiyasi va PCR inhibitorlarining ta’siri, RT-qPCR uchun RNK sifatiga qo‘yiladigan talablar, resurslari cheklangan laboratoriyalar uchun iqtisodiy samarali ekstraksiya usullari hamda SARS-CoV-2 diagnostikasidagi amaliy qo‘llanish jihatlari yoritildi. Bundan tashqari, ajratib olingan virus RNKsining genomik monitoring, mutatsiyalarni aniqlash, yangi avlod sekvensiyalash (NGS), diagnostik test tizimlari va ilmiy tadqiqotlarda tutgan o‘rni muhokama qilindi. Maqolada yuqori sifatli RNK olish imkonini beruvchi sodda, tezkor, arzon va avtomatlashtirishga mos texnologiyalarni rivojlantirish kelajakdagi epidemiyalar va pandemiyalarga tayyorgarlik ko‘rishda muhim ahamiyatga ega ekanligi ta’kidlangan.References
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Copyright (c) 2026 B. Turdaliyev Dilmurodjon, B. Sayidova Ra'no, O'. Allayev Otabek, B. Xoliyorova Feruza, Sh. Ashuraliyev Humoyun (Author)